Reporter

Part:BBa_K4799605:Design

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pNtNRAMP6_GUS_T-Nos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1097
    Illegal SpeI site found at 2228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4037
    Illegal SpeI site found at 2228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2019
    Illegal BamHI site found at 2002
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1097
    Illegal SpeI site found at 2228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1097
    Illegal SpeI site found at 2228
    Illegal NgoMIV site found at 2452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 41
    Illegal BsaI.rc site found at 251
    Illegal BsaI.rc site found at 2252
    Illegal BsaI.rc site found at 2867
    Illegal SapI.rc site found at 3038


Design Notes

Test if the promoter will induce GUS express after cadmium treatment.



Source

plasmid

References